Details

Autor(en) / Beteiligte

• Hu, Yanying
• Sun, Guojuan
• Zhang, Huitu
• Zhang, Liming
• Zhang, Tongcun
• Dai, Yujie
• Song, Cunjiang
• Liu, Hao
• Ram, Arthur

Titel

Determination of Ginkgolic Acids in Ginkgo Seeds Using HPLC-MS in the Presence of Lipids

Ist Teil von

• Advances in Applied Biotechnology, p.441-450

Ort / Verlag

Singapore: Springer Singapore

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• A method for determination of five kinds of ginkgolic acids in ginkgo seeds, C15:1, C17:2, C15:0, C17:1 using HPLC-MS was established. Isopropanol was employed as the extraction solvent and a component of the mobile phase. The isopropanol extract can be injected into the HPLC-MS apparatus directly and the lipid removal process with silica gel column chromatography can be saved. The limits of determination and quantification of this method are 0.977 and 0.293 mg kg−1 respectively. The coefficient of variation and the recovery of the determination method are 3.3 and 102.29% respectively. It has good linearity within the range of 0.0008–0.1 mg mL−1. This paper provides a simple and convenient method for the determination of ginkgolic acids in ginkgo seeds with the presence of a large amount of lipids.