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Detection of Dead Stained Microscopic Cells Based on Color Intensity and Contrast
Ist Teil von
The 1st International Conference on Advanced Intelligent System and Informatics (AISI2015), November 28-30, 2015, Beni Suef, Egypt, p.57-68
Ort / Verlag
Cham: Springer International Publishing
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
Apoptosis is an imperative constituent of various processes including proper progression and functioning of the immune system, embryonic development as well as chemical-induced cell death. Improper apoptosis is a reason in numerous human/animal’s conditions involving ischemic damage, neurodegenerative diseases, autoimmune disorders and various types of cancer. An outstanding feature of neurodegenerative diseases is the loss of specific neuronal populations. Thus, the detection of the dead cells is a necessity. This paper proposes a novel algorithm to achieve the dead cells detection based on color intensity and contrast changes and aims for fully automatic apoptosis detection based on image analysis method. A stained cultures images using Caspase stain of albino rats hippocampus specimens using light microscope (total 21 images) were used to evaluate the system performance. The results proved that the proposed system is efficient as it achieved high accuracy (98.89 ± 0.76 %) and specificity (99.36 ± 0.63 %) and good mean sensitivity level of (72.34 ± 19.85 %).