Details

Autor(en) / Beteiligte

• Reyes-Montes, M R
• Rodríguez-Arellanes, G
• Pérez-Torres, A
• Rosas-Rosas, A G
• Parás-García, A
• Juan-Sallés, C
• Taylor, M L

Titel

Identification of the source of histoplasmosis infection in two captive maras (Dolichotis patagonum) from the same colony by using molecular and immunologic assays

Ist Teil von

• Revista argentina de microbiología, 2009-04, Vol.41 (2), p.102-104

Ort / Verlag

Argentina: Asociación Argentina de Microbiología

Erscheinungsjahr

2009

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Histoplasma capsulatum was isolated from the spleen of a first infected mara (Dolichotis patagonum) and from a second mara's liver and adrenal gland, both in the same colony at the Africam Safari, Puebla, Mexico. Studies of H. capsulatum isolates, using nested-PCR of a 100-kDa protein coding gene (Hcp100) fragment and a two-primer RAPD-PCR method, suggest that these isolates were spreading in the environment of the maras' enclosure. By using a Dot-ELISA method, sera from mice inoculated with three homogenates of soil samples from the maras' enclosed space developed positive brown spot reactions to a purified H. capsulatum antigen, which identified the probable source of the maras' infection.