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Details

Autor(en) / Beteiligte
Titel
Optimization of visibility and quantification of aberrant crypt foci in colonic mucosa in Wistar rats
Ist Teil von
  • Acta Cirurgica Brasileira, 2010-04, Vol.25 (2), p.148-152
Ort / Verlag
Brazil: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Erscheinungsjahr
2010
Quelle
MEDLINE
Beschreibungen/Notizen
  • Test immersion of microscopy samples in water as an aid to visualizing and quantifying aberrant crypt foci (ACF) in rat colon mucosa. Carcinogenesis was induced with azoxymethane in Wistar rats kept on a conventional diet or a hypercaloric diet containing unsaturated fat. Fifteen weeks after induction, colon samples were retrieved and fixated in a 10% formaldehyde solution. The samples were divided into segments (distal, middle, proximal) and stained with 1% toluidine blue. The technique tested in the study consisted of immersing microscopy samples in distilled water in order to eliminate the problem of light reflection known from conventional microscopy. When samples were immersed in water during microscopy, significantly more ACF could be visualized in all colon segments than with the conventional method proposed by Bird. Immersing microscopy samples in water aids the visualization and quantification of aberrant crypt foci in rat colon mucosa fixed in formaldehyde.
Sprache
Englisch; Portugiesisch
Identifikatoren
ISSN: 0102-8650, 1678-2674
eISSN: 1678-2674, 0102-8650
DOI: 10.1590/S0102-86502010000200005
Titel-ID: cdi_scielo_journals_S0102_86502010000200005

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