Details

Autor(en) / Beteiligte

• Mazzetti, Marcelo Paulo Vaccari
• Oliveira, Isis Sousa
• Miranda-Ferreira, Regiane
• Fauaz, Grasiele
• Ribeiro, Chaibe Nunes
• Gomes, Paulo Oliveira
• Pontes, Paulo
• Ferreira, Alice Teixeira
• Eça, Lilian Piñero

Titel

Qualitative and quantitative analysis of rabbit's fat mesenchymal stem cells

Ist Teil von

• Acta Cirurgica Brasileira, 2010-02, Vol.25 (1), p.24-27

Ort / Verlag

Brazil: Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia

Erscheinungsjahr

2010

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• To present an experimental model of qualitative and quantitative analysis of mesenchymal stem cells from fat of rabbits obtained by lipectomy. The fat could be a great source for obtaining mesenchymal stem cells and to create conditions for repairing injured tissues by bioengineering. New Zealand rabbits (n = 10) adipose panicle (2-3 cm) were removed by lipectomy, fragmented and washed with PBS and enzymatically dissociated with trypsin/EDTA. Lately, these cells were incubated in culture medium DMEM and after 20 days, was performed quantitative analysis of the accession of first and second mesenchymal cells in cell culture bottles. The fat total cells (CTF) were 1.62 x10(6) cells/mL and presented 98% of viability. These cells were taken for cultivation and after 20 days were counted 2.88 x10(6) cells/mL MSC. The same was done and after 20 days we quantified 4.28 x10(6) cells/mL MSC. The lipectomy of adipose panicule is a very satisfactory method to extract stem cells from fat, quantitatively and qualitatively.