Details

Autor(en) / Beteiligte

• Rodriguez-Moncayo, Roberto
• Cedillo-Alcantar, Diana F
• Guevara-Pantoja, Pablo E
• Chavez-Pineda, Oriana G
• Hernandez-Ortiz, Jose A
• Amador-Hernandez, Josue U
• Rojas-Velasco, Gustavo
• Sanchez-Muñoz, Fausto
• Manzur-Sandoval, Daniel
• Patino-Lopez, Luis D
• May-Arrioja, Daniel A
• Posadas-Sanchez, Rosalinda
• Vargas-Alarcon, Gilberto
• Garcia-Cordero, Jose L

Titel

A high-throughput multiplexed microfluidic device for COVID-19 serology assays

Ist Teil von

• Lab on a chip, 2021-01, Vol.21 (1), p.93-14

Ort / Verlag

England: Royal Society of Chemistry

Erscheinungsjahr

2021

Quelle

MEDLINE

Beschreibungen/Notizen

• The applications of serology tests to the virus SARS-CoV-2 are diverse, ranging from diagnosing COVID-19, understanding the humoral response to this disease, and estimating its prevalence in a population, to modeling the course of the pandemic. COVID-19 serology assays will significantly benefit from sensitive and reliable technologies that can process dozens of samples in parallel, thus reducing costs and time; however, they will also benefit from biosensors that can assess antibody reactivities to multiple SARS-CoV-2 antigens. Here, we report a high-throughput microfluidic device that can assess antibody reactivities against four SARS-CoV-2 antigens from up to 50 serum samples in parallel. This semi-automatic platform measures IgG and IgM levels against four SARS-CoV-2 proteins: the spike protein (S), the S1 subunit (S1), the receptor-binding domain (RBD), and the nucleocapsid (N). After assay optimization, we evaluated sera from infected individuals with COVID-19 and a cohort of archival samples from 2018. The assay achieved a sensitivity of 95% and a specificity of 91%. Nonetheless, both parameters increased to 100% when evaluating sera from individuals in the third week after symptom onset. To further assess our platform's utility, we monitored the antibody titers from 5 COVID-19 patients over a time course of several weeks. Our platform can aid in global efforts to control and understand COVID-19. A microfluidic device to quantitate antibody reactivities to SARS-CoV-2 antigens: spike protein, RDB, S1 subunit, and nucleocapsid, from 50 serum samples.