Details

Autor(en) / Beteiligte

• Jung, Ye Lim
• Lee, Chang Yeol
• Park, Jung Hun
• Park, Ki Soo
• Park, Hyun Gyu

Titel

A signal-on, colorimetric determination of deoxyribonuclease I activity utilizing the photoinduced synthesis of gold nanoparticlesElectronic supplementary information (ESI) available. See DOI: 10.1039/c7nr09542b

Erscheinungsjahr

2018-03

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• A simple, colorimetric method is developed for the determination of deoxyribonuclease I (DNase I) activity based on the novel finding that DNase I can promote the photoinduced synthesis of gold nanoparticles (AuNPs). In the absence of DNase I, a phosphorothioate (PS) DNA probe remains intact and captures Au( iii ) through a strong Au-thiol interaction, which prevents the photoinduced synthesis of AuNPs, leaving the sample in a colorless state. On the other hand, in the presence of DNase I, the PS DNA probe is cleaved into small fragments that are removed via a simple purification process. The resulting solution, after the incubation with HAuCl 4 and threonine (Thr), forms AuNPs by UV light irradiation with the aid of Thr which acts as a catalyst for the Au( iii ) reduction process. As a result, a red-colored suspension is produced. By monitoring the color changes of the samples with the naked eye, the DNase I activity was conveniently determined. In addition, the clinical utility of this simple, yet highly efficient colorimetric strategy was verified by reliably quantifying the DNase I activities in a bovine urine sample. Importantly, the working principle designed for the determination of DNase I activity was successfully expanded for the detection of target nucleic acids, ensuring the universal applicability of the developed assay system. A signal-on, colorimetric deoxyribonuclease I (DNase I) activity assay is developed based on the novel finding that DNase I can promote the photoinduced synthesis of gold nanoparticles.