Details

Autor(en) / Beteiligte

• Bhat, R
• García, I
• Aznar, E
• Arnaiz, B
• Martínez-Bisbal, M. C
• Liz-Marzán, L. M
• Penadés, S
• Martínez-Máñez, R

Titel

Lectin-gated and glycan functionalized mesoporous silica nanocontainers for targeting cancer cells overexpressing Lewis X antigenElectronic supplementary information (ESI) available: PXRD patterns of the as-synthesized MSNs and extracted MSNs; TEM images of MSNs and solid S3; N2 adsorption-desorption isotherms for MSNs and pore size distribution; and the release profile of ATTO 430LS dye from solid S3 in cellular medium. See DOI: 10.1039/c7nr06415b

Erscheinungsjahr

2017-12

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Gated mesoporous silica nanoparticles can deliver payload upon the application of a predefined stimulus, and therefore are promising drug delivery systems. Despite their important role, relatively low emphasis has been placed on the design of gating systems that actively target carbohydrate tumor cell membrane receptors. We describe herein a new Lewis X (Le x ) antigen-targeted delivery system comprising mesoporous silica nanoparticles (MSNs) loaded with ATTO 430LS dye, functionalized with a Le x derivative ( 1 ) and capped with a fucose-specific carbohydrate-binding protein ( Aleuria aurantia lectin (AAL)). This design takes advantage of the affinity of AAL for Le x overexpressed receptors in certain cancer cells. In the proximity of the cells, AAL is detached from MSNs to bind Le x , and selectins in the cells bind Le x in the gated MSNs, thereby inducing cargo delivery. Gated MSNs are nontoxic to colon cancer DLD-1 cells, and ATTO 430LS dye delivered correlated with the amount of Le x antigen overexpressed at the DLD-1 cell surface. This is one of the few examples of MSNs using biologically relevant glycans for both capping ( via interaction with AAL) and targeting ( via interaction with overexpressed Le x at the cell membrane). Gated MSNs using biologically relevant glycans for both capping and targeting.