Details

Autor(en) / Beteiligte

• Watanabe, R
• Soga, N
• Hara, M
• Noji, H

Titel

Arrayed water-in-oil droplet bilayers for membrane transport analysisElectronic supplementary information (ESI) available. See DOI: 10.1039/c6lc00155f

Erscheinungsjahr

2016-08

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The water-in-oil droplet bilayer is a simple and useful lipid bilayer system for membrane transport analysis. The droplet interface bilayer is readily formed by the contact of two water-in-oil droplets enwrapped by a phospholipid monolayer. However, the size of individual droplets with femtoliter volumes in a high-throughput manner is difficult to control, resulting in low sensitivity and throughput of membrane transport analysis. To overcome this drawback, in this study, we developed a novel micro-device in which a large number of droplet interface bilayers (>500) are formed at a time by using femtoliter-sized droplet arrays immobilized on a hydrophobic/hydrophilic substrate. The droplet volume was controllable from 3.5 to 350 fL by changing the hydrophobic/hydrophilic pattern on the device, allowing high-throughput analysis of membrane transport mechanisms including membrane permeability to solutes ( e.g. , ions or small molecules) with or without the aid of transport proteins. Thus, this novel platform broadens the versatility of water-in-oil droplet bilayers and will pave the way for novel analytical and pharmacological applications such as drug screening. We developed an arrayed water-in-oil droplet bilayer system for highly sensitive and quantitative membrane transport analysis.