Details

Autor(en) / Beteiligte

• Rodríguez-Pulido, Alberto
• Cortajarena, Aitziber L
• Torra, Joaquim
• Ruiz-González, Rubén
• Nonell, Santi
• Flors, Cristina

Titel

Assessing the potential of photosensitizing flavoproteins as tags for correlative microscopyElectronic supplementary information (ESI) available: Materials and methods, Fig. S1 and S2. See DOI: 10.1039/c6cc03119f

Erscheinungsjahr

2016-06

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Photosensitizing flavoproteins have great potential as tags for correlative light and electron microscopy (CLEM). We examine the photostability of miniSOG mutants and their ability to photo-oxidize diaminobenzidine, both key aspects for CLEM. Our experiments reveal a complex relation between these parameters and the production of different reactive oxygen species. A complex relation is found between the photosensitization properties of flavoproteins and their potential for correlative microscopy.