Details

Autor(en) / Beteiligte

• Pang, Lanfang
• Zhou, Yanmei
• Wang, Enze
• Yu, Fang
• Zhou, Hua
• Gao, Wenli

Titel

A "turn-on" fluorescent probe used for the specific recognition of intracellular GSH and its application in bioimagingElectronic supplementary information (ESI) available. See DOI: 10.1039/c5ra25204k

Erscheinungsjahr

2016-02

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• We have designed and synthesized a carbazole-based fluorescent probe (CZ-Nm) for the specific recognition of GSH over Cys and Hcy. The probe is almost non-fluorescent owing to the photoinduced electron transfer (PET) process from the carbazole fluorophore to the nitroolefin moiety. Upon treatment with GSH, owing to the Michael addition of GSH to the double bond of the nitroolefin moiety, the probe shows a fluorescence enhancement and absorption change. The CZ-Nm probe displays desirable properties such as acting as a "naked eye" probe, a wide linear range of 0-0.01 M, high sensitivity, and strong anti-jamming capability. More importantly, the probe can also be successfully applied to the detection of intracellular GSH with a bright fluorescence signal, good cell permeability and bio-compatibility. A "turn-on" fluorescent probe used to specifically recognize intracellular GSH and its application in bioimaging.