Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Finding a suitable separation condition for TLC/FTIR analysis by using multiple-narrow-band TLC technique
Ist Teil von
RSC advances, 2015-01, Vol.5 (28), p.21544-21549
Erscheinungsjahr
2015
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
Thin layer chromatography (TLC) coupled with infrared spectroscopic technique has great advantages in the separation and identification of different components in a mixture. However, determination of suitable separation condition is a tedious task. To improve the efficiency of finding suitable separation conditions, we propose a multi-narrow-band TLC approach. In this approach, a TLC plate containing several parallel narrow bands was utilized. In experiment, different pure substance was added on each of the narrow bands and TLC analysis was performed under same condition. Different substance exhibits different migration distance. Thus, it becomes much easier to separate substances with large difference in migration distance. This approach can be used to find multiple mixed sample pairs in a parallel manner. Consequently, a high-throughput method on selection of suitable mobile phase and separation conditions can be established.
A multiple-narrow-band TLC approach is proposed to find suitable solvent for TLC analysis in a high-throughput manner.