Details

Autor(en) / Beteiligte

• Yu, Songrui
• Chen, Jianxin
• Dong, Ruijiao
• Su, Yue
• Ji, Bing
• Zhou, Yongfeng
• Zhu, Xinyuan
• Yan, Deyue

Titel

Enhanced gene transfection efficiency of PDMAEMA by incorporating hydrophobic hyperbranched polymer cores: effect of degree of branchingElectronic supplementary information (ESI) available: The synthesis scheme of PEHO-g-PDMAEMAs, characterizations of PEHOs and PEHO-g-PDMAEMAs, Zeta potential data of PEHO-g-PDMAEMAs. See DOI: 10.1039/c2py20487h

Erscheinungsjahr

2012-10

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) is a well-known cationic polymer candidate for non-viral vectors for gene transfection. However, such an application has been greatly limited due to the cytotoxicity of the polymers. Herein, PDMAEMAs are grafted from hydrophobic hyperbranched PEHO cores (PEHO means poly(3-ethyl-3-(hydroxymethyl)-oxetane)), and the obtained hyperbranched multiarm copolymers of PEHO- g -PDMAEMAs show higher transfection efficiency than that of branched polyethylenimine (PEI) and PDMAEMA homopolymers, due to the improved cytotoxicity, DNA compaction, buffering ability and cellular uptake. In addition, to disclose the structure-property relationship, a series of PEHO- g -PDMAEMAs with different topological architectures are synthesized by changing the degrees of branching (DBs) of the PEHO cores and the lengths of the PDMAEMA arms. The ability of these vectors in DNA compaction, buffering ability, cytotoxicity and gene transfection efficiency is also investigated. It has been found the gene transfection efficiency of the vectors is dependent on the DB of the PEHO cores, but almost independent of the PDMAEMA arms in the experimental range. The work reports the gene transfection behaviors of PDMAEMAs incorporated with a hydrophobic hyperbranched core and a star structure.