Details

Autor(en) / Beteiligte

• Lee, Beom Seok
• Lee, Yang Ui
• Kim, Han-Sang
• Kim, Tae-Hyeong
• Park, Jiwoon
• Lee, Jeong-Gun
• Kim, Jintae
• Kim, Hanshin
• Lee, Wee Gyo
• Cho, Yoon-Kyoung

Titel

Fully integrated lab-on-a-disc for simultaneous analysis of biochemistry and immunoassay from whole bloodElectronic supplementary information (ESI) available: Movie file and caption. See DOI: 10.1039/c0lc00205dPublished as part of a LOC themed issue dedicated to Korean Research: Guest Editors: Professor Je-Kyun Park and Kahp-Yang Suh

Erscheinungsjahr

2010-12

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• We report a fully integrated device that can perform both multiple biochemical analysis and sandwich type immunoassay simultaneously on a disc. The whole blood is applied directly to the disposable "lab-on-a-disc" containing different kinds of freeze-dried reagents for the blood chemistry analysis as well as reagents required for the immunoassay. The concentrations of different kinds of analytes are reported within 22 min by simply inserting a disc to a portable device. Using the innovative laser irradiated ferrowax microvalves together with the centrifugal microfluidics, the total process of plasma separation, metering, mixing, incubation, washing, and detection is fully automated. The analyzer is equipped with an optical detection module to measure absorbances at 10 different wavelengths to accommodate the various kinds of reaction protocols. Compared to the conventional blood analysis done in clinical laboratories, it is advantageous for point-of-care applications because it requires a smaller amount of blood (350 μL vs. 3 mL), takes less time (22 min vs. several days), does not require specially trained operators or expensive instruments to run biochemical analysis and immunoassay separately. We report a fully integrated lab-on-a-disc system for simultaneous analysis of clinical chemistry and enzyme-linked immuno-sorbent assay (ELISA) from whole blood.