Details

Autor(en) / Beteiligte

• Shan, Bo
• Barker, Clive S.
• Shao, Mengle
• Zhang, Qianbin
• Gupta, Rana K.
• Wu, Yibo

Titel

Multilayered omics reveal sex- and depot-dependent adipose progenitor cell heterogeneity

Ist Teil von

• Cell metabolism, 2022-05, Vol.34 (5), p.783-799.e7

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2022

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Single-cell RNA sequencing (scRNA-seq) has revealed that adult white adipose tissue (WAT) harbors functionally diverse subpopulations of mesenchymal stromal cells that differentially impact tissue plasticity. To date, the molecular basis of this cellular heterogeneity has not been fully defined. Here, we describe a multilayered omics approach to dissect adipose progenitor cell heterogeneity in three dimensions: progenitor subpopulation, sex, and anatomical localization. We applied state-of-the-art mass spectrometry methods to quantify 4,870 proteins in eight different stromal cell populations from perigonadal and inguinal WAT of male and female mice and acquired transcript expression levels of 15,477 genes using RNA-seq. Our data unveil molecular signatures defining sex differences in preadipocyte differentiation and identify regulatory pathways that functionally distinguish adipose progenitor subpopulations. This multilayered omics analysis, freely accessible at http://preadprofiler.net/, provides unprecedented insights into adipose stromal cell heterogeneity and highlights the benefit of complementary proteomics to support findings from scRNA-seq studies. [Display omitted] •Integrated multilayer omics reveal molecular heterogeneity of adipose progenitors•Proteomic analysis highlights metabolic heterogeneity of adipose progenitors•PPARγ phosphorylation underlies sex differences in iWAT APC differentiation•Glutathione metabolism and AhR signaling regulate progenitor cell fate and function Adipose tissue harbors functionally distinct progenitor cell subpopulations. Shan et al. utilize multilayered omics to dissect adipose progenitor cell heterogeneity in three dimensions: progenitor subpopulation, sex, and anatomical localization. Functional analysis unveils mechanisms underlying sex differences in preadipocyte activation and pathways controlling the adipogenic and pro-inflammatory properties of distinct progenitor subpopulations.