Details

Autor(en) / Beteiligte

• Zhang, Shaohua
• Zhang, Qianyu
• Liu, Zixin
• Liu, Kuo
• He, Lingjuan
• Lui, Kathy O
• Wang, Lixin
• Zhou, Bin

Titel

Genetic dissection of intercellular interactions in vivo by membrane-permeable protein

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 2023-01, Vol.120 (1), p.e2120582120-e2120582120

Ort / Verlag

United States: National Academy of Sciences

Erscheinungsjahr

2023

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Unraveling cell-cell interaction is fundamental to understanding many biological processes. To date, genetic tools for labeling neighboring cells in mammals are not available. Here, we developed a labeling strategy based on the Cre-induced intercellular labeling protein (CILP). Cre-expressing donor cells release a lipid-soluble and membrane-permeable fluorescent protein that is then taken up by recipient cells, enabling fluorescent labeling of neighboring cells. Using CILP, we specifically labeled endothelial cells surrounding a special population of hepatocytes in adult mice and revealed their distinct gene signatures. Our results highlight the potential of CILP as a platform to reveal cell-cell interactions and communications in vivo.