Details
- Autor(en) / Beteiligte
- Titel
- Recent advances in genetic tools for engineering probiotic lactic acid bacteria
- Ist Teil von
- Bioscience reports, 2023-01, Vol.43 (1), p.1
- Ort / Verlag
- England: Portland Press Ltd The Biochemical Society
- Erscheinungsjahr
- 2023
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Synthetic biology has grown exponentially in the last few years, with a variety of biological applications. One of the emerging applications of synthetic biology is to exploit the link between microorganisms, biologics, and human health. To exploit this link, it is critical to select effective synthetic biology tools for use in appropriate microorganisms that would address unmet needs in human health through the development of new game-changing applications and by complementing existing technological capabilities. Lactic acid bacteria (LAB) are considered appropriate chassis organisms that can be genetically engineered for therapeutic and industrial applications. Here, we have reviewed comprehensively various synthetic biology techniques for engineering probiotic LAB strains, such as clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 mediated genome editing, homologous recombination, and recombineering. In addition, we also discussed heterologous protein expression systems used in engineering probiotic LAB. By combining computational biology with genetic engineering, there is a lot of potential to develop next-generation synthetic LAB with capabilities to address bottlenecks in industrial scale-up and complex biologics production. Recently, we started working on Lactochassis project where we aim to develop next generation synthetic LAB for biomedical application.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0144-8463eISSN: 1573-4935DOI: 10.1042/BSR20211299Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9842951
- Format
- –
- Schlagworte
- Antigens, Antimicrobial agents, Bacteria, Bioinformatics, Biological effects, Biological Products, Biology, Biomedical materials, Biotechnology, Cancer therapies, CRISPR, Cytokines, Diabetes, Disease prevention, Drug delivery systems, Exploitation, Gene Editing - methods, Genetic engineering, Genetic Engineering - methods, Genomes, HIV, Homologous recombination, Human immunodeficiency virus, Human papillomavirus, Humans, Immune system, Industrial applications, Infectious diseases, Inflammatory bowel disease, Influenza, Lactic acid, Lactic acid bacteria, Lactobacillales - genetics, Leprosy, Listeria, Metabolism, Metabolites, Microorganisms, Pathogens, Peptides, Probiotics, Probiotics - therapeutic use, Proteins, Recombination, Review, Synthetic Biology, Vectors (Biology)