Details

Autor(en) / Beteiligte

• Banahene, Nicholas
• Gepford, Dana M.
• Biegas, Kyle J.
• Swanson, Daniel H.
• Hsu, Yen‐Pang
• Murphy, Brennan A.
• Taylor, Zachary E.
• Lepori, Irene
• Siegrist, M. Sloan
• Obregón‐Henao, Andrés
• Van Nieuwenhze, Michael S.
• Swarts, Benjamin M.

Titel

A Far‐Red Molecular Rotor Fluorogenic Trehalose Probe for Live Mycobacteria Detection and Drug‐Susceptibility Testing

Ist Teil von

• Angewandte Chemie International Edition, 2023-01, Vol.62 (2), p.e202213563-n/a

Auflage

International ed. in English

Ort / Verlag

Germany: Wiley Subscription Services, Inc

Erscheinungsjahr

2023

Quelle

MEDLINE

Beschreibungen/Notizen

• Increasing the speed, specificity, sensitivity, and accessibility of mycobacteria detection tools are important challenges for tuberculosis (TB) research and diagnosis. In this regard, previously reported fluorogenic trehalose analogues have shown potential, but their green‐emitting dyes may limit sensitivity and applications in complex settings. Here, we describe a trehalose‐based fluorogenic probe featuring a molecular rotor turn‐on fluorophore with bright far‐red emission (RMR‐Tre). RMR‐Tre, which exploits the unique biosynthetic enzymes and environment of the mycobacterial outer membrane to achieve fluorescence activation, enables fast, no‐wash, low‐background fluorescence detection of live mycobacteria. Aided by the red‐shifted molecular rotor fluorophore, RMR‐Tre exhibited up to a 100‐fold enhancement in M. tuberculosis labeling compared to existing fluorogenic trehalose probes. We show that RMR‐Tre reports on M. tuberculosis drug resistance in a facile assay, demonstrating its potential as a TB diagnostic tool. The development of a novel trehalose‐based fluorogenic probe that features a molecular rotor turn‐on fluorophore with exceptionally bright far‐red emission is described (RMR‐Tre). RMR‐Tre enables rapid, no‐wash, low‐background fluorescence detection and drug‐susceptibility evaluation of live mycobacteria, including Mycobacterium tuberculosis.