Details

Autor(en) / Beteiligte

• McGinniss, John E
• Whiteside, Samantha A
• Deek, Rebecca A
• Simon-Soro, Aurea
• Graham-Wooten, Jevon
• Oyster, Michelle
• Brown, Melanie D
• Cantu, Edward
• Diamond, Joshua M
• Li, Hongzhe
• Christie, Jason D
• Bushman, Frederic D
• Collman, Ronald G

Titel

The Lung Allograft Microbiome Associates with Pepsin, Inflammation, and Primary Graft Dysfunction

Ist Teil von

• American journal of respiratory and critical care medicine, 2022-12, Vol.206 (12), p.1508-1521

Ort / Verlag

United States: American Thoracic Society

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Primary graft dysfunction (PGD) is the principal cause of early morbidity and mortality after lung transplantation. The lung microbiome has been implicated in later transplantation outcomes but has not been investigated in PGD. To define the peritransplant bacterial lung microbiome and relationship to host response and PGD. This was a single-center prospective cohort study. Airway lavage samples from donor lungs before organ procurement and recipient allografts immediately after implantation underwent bacterial 16S ribosomal ribonucleic acid gene sequencing. Recipient allograft samples were analyzed for cytokines by multiplex array and pepsin by ELISA. We enrolled 139 transplant subjects and obtained donor lung (  = 109) and recipient allograft (  = 136) samples. Severe PGD (persistent grade 3) developed in 15 subjects over the first 72 hours, and 40 remained without PGD (persistent grade 0). The microbiome of donor lungs differed from healthy lungs, and recipient allograft microbiomes differed from donor lungs. Development of severe PGD was associated with enrichment in the immediate postimplantation lung of oropharyngeal anaerobic taxa, particularly . Elevated pepsin, a gastric biomarker, and a hyperinflammatory cytokine profile were present in recipient allografts in severe PGD and strongly correlated with microbiome composition. Together, immediate postimplantation allograft / ratio, pepsin, and indicator cytokines were associated with development of severe PGD during the 72-hour post-transplantation period (area under the curve = 0.81). Lung allografts that develop PGD have a microbiome enriched in anaerobic oropharyngeal taxa, elevated gastric pepsin, and hyperinflammatory phenotype. These findings suggest a possible role for peritransplant aspiration in PGD, a potentially actionable mechanism that warrants further investigation.