Details

Autor(en) / Beteiligte

• Ariga, Yuzuru
• Low, Shulin
• Hoshino, Hitomi
• Nakada, Tsutomu
• Akama, Tomoya O.
• Muramoto, Akifumi
• Fukushima, Mana
• Yamauchi, Takahiro
• Ohshima, Yusei
• Kobayashi, Motohiro

Titel

Expression and Clinical Significance of Spi-B in B-cell Acute Lymphoblastic Leukemia

Ist Teil von

• The journal of histochemistry and cytochemistry, 2022-10, Vol.70 (10), p.683-694

Ort / Verlag

Los Angeles, CA: SAGE Publications

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Spi-B, a member of the E26 transformation-specific (ETS) family of transcription factors, plays an important role in B cell differentiation. Spi-B also functions in development of diffuse large B-cell lymphoma; thus, we hypothesized that it may participate in leukemogenesis of B-cell acute lymphoblastic leukemia (B-ALL). To test this hypothesis, we first generated an anti-Spi-B monoclonal antibody that recognized Spi-B on formalin-fixed, paraffin-embedded tissue sections. This antibody, designated S28-5, selectively stained B cell nuclei at the pre-plasma cell stage (including centrocytes and centroblasts in germinal centers) and nuclei of plasmacytoid dendritic cells, but not fully differentiated plasma cells, T cells, macrophages, or follicular dendritic cells. Employing S28-5, we then performed immunohistochemical staining of bone marrow aspiration biopsy specimens obtained from B-ALL patients (n=62). Cases that showed stronger nuclear S28-5 signals than T-cell ALL were scored positive. In 26 (42%) of 62 specimens, leukemic cells showed nuclear Spi-B expression, and positivity was associated with patient age at diagnosis, and serum uric acid and creatinine levels. Moreover, Spi-B-positive patients demonstrated significantly shorter overall survival than did Spi-B-negative patients. These results suggest that Spi-B expression may serve as a prognostic indicator of B-ALL.