Proceedings of the National Academy of Sciences - PNAS, 2022-11, Vol.119 (44), p.e2210434119-e2210434119
2022
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- Autor(en) / Beteiligte
- Titel
- Phosphorylation of RXRα mediates the effect of JNK to suppress hepatic FGF21 expression and promote metabolic syndrome
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 2022-11, Vol.119 (44), p.e2210434119-e2210434119
- Ort / Verlag
- United States: National Academy of Sciences
- Erscheinungsjahr
- 2022
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The cJun NH -terminal kinase (JNK) signaling pathway in the liver promotes systemic changes in metabolism by regulating peroxisome proliferator-activated receptor α (PPARα)-dependent expression of the hepatokine fibroblast growth factor 21 (FGF21). Hepatocyte-specific gene ablation studies demonstrated that the gene (encoding JNK2) plays a key mechanistic role. Mutually exclusive inclusion of exons 7a and 7b yields expression of the isoforms JNK2α and JNK2β. Here we demonstrate that gene expression and metabolic regulation are primarily regulated by the JNK2α isoform. To identify relevant substrates of JNK2α, we performed a quantitative phosphoproteomic study of livers isolated from control mice, mice with JNK deficiency in hepatocytes, and mice that express only JNK2α or JNK2β in hepatocytes. We identified the JNK substrate retinoid X receptor α (RXRα) as a protein that exhibited JNK2α-promoted phosphorylation in vivo. RXRα functions as a heterodimeric partner of PPARα and may therefore mediate the effects of JNK2α signaling on expression. To test this hypothesis, we established mice with hepatocyte-specific expression of wild-type or mutated RXRα proteins. We found that the RXRα phosphorylation site Ser was required for suppression of gene expression. Collectively, these data establish a JNK-mediated signaling pathway that regulates hepatic expression.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0027-8424eISSN: 1091-6490DOI: 10.1073/pnas.2210434119Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9636906
- Format
- –
- Schlagworte
- Ablation, Animals, Biological Sciences, Carrier Proteins - metabolism, Exons, Fibroblast growth factors, Fibroblast Growth Factors - metabolism, Gene expression, Growth factors, Hepatocytes, Hepatocytes - metabolism, In vivo methods and tests, Isoforms, Kinases, Liver, Liver - metabolism, MAP Kinase Kinase 4 - metabolism, Metabolic disorders, Metabolic syndrome, Metabolic Syndrome - metabolism, Metabolism, Mice, Mice, Knockout, Peroxisome proliferator-activated receptors, Phosphorylation, PPAR alpha - genetics, PPAR alpha - metabolism, Proteins, Receptors, Retinoid X Receptor alpha - genetics, Retinoid X Receptor alpha - metabolism, Signal transduction, Signaling, Substrates