Details

Autor(en) / Beteiligte

• Sherief, Dalia
• Hassan, Asmaa
• Habeeb, Eman
• Nosair, Nahla
• Mabrouk, Maaly
• Shoeib, Sarah
• Reyad, Heba
• Haydra, Tamer
• Watany, Mona

Titel

High-resolution Melting Analysis for NOTCH1 c.7541-7542delCT Mutation in Chronic Lymphocytic Leukemia: Prognostic Significance in Egyptian Patients

Ist Teil von

• Indian journal of hematology & blood transfusion, 2022-10, Vol.38 (4), p.675-679

Ort / Verlag

New Delhi: Springer India

Erscheinungsjahr

2022

Quelle

SpringerLink Journals

Beschreibungen/Notizen

• The present study aimed to detect the prevalence of NOTCH1 c.7541-7542delCT mutation in Egyptian CLL patients using HRM assay and to assess its relation to patients’ survival. The study included 50 newly diagnosed treatment-naïve CLL patients and 50 age and sex matched healthy controls. NOTCH1 c.7541-7542delCT mutation was detected using High-resolution melting (HRM) assay and direct Sanger sequencing. Outcome parameters included progression free survival (PFS) and overall survival (OS). NOTCH1 c.7541-7542delCT mutation was detected in 5 (10.0%) of CLL patients. No controls had NOTCH1 c.7541-7542delCT mutation. Similar results were obtained by direct Sanger sequencing yielding a sensitivity and specificity of 100.0% for HRM in detection of NOTCH1 c.7541-7542delCT mutation in the studied patients. In univariate analysis, predictors of OS included Trisomy 12, high LDH, presence of NOTCH1 c.7541-7542delCT mutation and lack of CR. In multivariate analysis, only lack of CR was found as a significant predictor of OS. HRM analysis is a sensitive method for detection of NOTCH1 c.7541-7542delCT mutation in CLL patients. This mutation may be linked to poor disease prognosis.