Details

Autor(en) / Beteiligte

• Wagt, Sander
• de Haan, Noortje
• Wang, Wenjun
• Zhang, Tao
• Wuhrer, Manfred
• Lageveen-Kammeijer, Guinevere S. M.

Titel

N‑Glycan Isomer Differentiation by Zero Flow Capillary Electrophoresis Coupled to Mass Spectrometry

Ist Teil von

• Analytical chemistry (Washington), 2022-09, Vol.94 (38), p.12954-12959

Ort / Verlag

Washington: American Chemical Society

Erscheinungsjahr

2022

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Isomeric N-glycans often vastly differ in their biological activities, hence the need for methods that allow resolving and structurally characterizing them in biological material. Here, we established a zero flow approach using capillary electrophoresis in combination with (tandem) mass spectrometry to allow structural characterization of isomeric N-glycans at high sensitivity. Additionally, diagnostic fragment ion ratios were identified, indicative for the antenna carrying specifically linked sialic acids. In total, 208 N-glycans were characterized in human plasma, with 57 compositions showing multiple isomers.