Details

Autor(en) / Beteiligte

• Torabi, Seyed-Fakhreddin
• Vaidya, Anand T
• Tycowski, Kazimierz T
• DeGregorio, Suzanne J
• Wang, Jimin
• Shu, Mei-Di
• Steitz, Thomas A
• Steitz, Joan A

Titel

RNA stabilization by a poly(A) tail 3'-end binding pocket and other modes of poly(A)-RNA interaction

Ist Teil von

• Science (American Association for the Advancement of Science), 2021-02, Vol.371 (6529)

Ort / Verlag

United States: The American Association for the Advancement of Science

Erscheinungsjahr

2021

Link zum Volltext

Quelle

American Association for the Advancement of Science

Beschreibungen/Notizen

• Polyadenylate [poly(A)] tail addition to the 3' end of a wide range of RNAs is a highly conserved modification that plays a central role in cellular RNA function. Elements for nuclear expression (ENEs) are cis-acting RNA elements that stabilize poly(A) tails by sequestering them in RNA triplex structures. A crystal structure of a double ENE from a rice hAT transposon messenger RNA complexed with poly(A) at a resolution of 2.89 angstroms reveals multiple modes of interaction with poly(A), including major-groove triple helices, extended minor-groove interactions with RNA double helices, a quintuple-base motif that transitions poly(A) from minor-groove associations to major-groove triple helices, and a poly(A) 3'-end binding pocket. Our findings both expand the repertoire of motifs involved in long-range RNA interactions and provide insights into how polyadenylation can protect an RNA's extreme 3' end.