Details

Autor(en) / Beteiligte

• Fukui, Kosuke
• Arai, Kazushi
• Tanaka, Yuka
• Aoi, Yuki
• Kukshal, Vandna
• Jez, Joseph M
• Kubes, Martin F
• Napier, Richard
• Zhao, Yunde
• Kasahara, Hiroyuki
• Hayashi, Ken-Ichiro

Titel

Chemical inhibition of the auxin inactivation pathway uncovers the roles of metabolic turnover in auxin homeostasis

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 2022-08, Vol.119 (32), p.e2206869119

Ort / Verlag

United States: National Academy of Sciences

Erscheinungsjahr

2022

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• The phytohormone auxin, indole-3-acetic acid (IAA), plays a prominent role in plant development. Auxin homeostasis is coordinately regulated by auxin synthesis, transport, and inactivation; however, the physiological contribution of auxin inactivation to auxin homeostasis has not been determined. The GH3 IAA-amino acid conjugating enzymes play a central role in auxin inactivation. Chemical inhibition of GH3 proteins is challenging because the inhibition of these enzymes leads to IAA overaccumulation that rapidly induces expression. Here, we report the characterization of a potent GH3 inhibitor, kakeimide, that selectively targets IAA-conjugating GH3 proteins. Chemical knockdown of the auxin inactivation pathway demonstrates that auxin turnover is very rapid (about 10 min) and indicates that both auxin biosynthesis and inactivation dynamically regulate auxin homeostasis.