Details

Autor(en) / Beteiligte

• Turner, Nicholas L.
• Macrina, Thomas
• Bae, J. Alexander
• Yang, Runzhe
• Wilson, Alyssa M.
• Schneider-Mizell, Casey
• Lee, Kisuk
• Lu, Ran
• Wu, Jingpeng
• Bodor, Agnes L.
• Bleckert, Adam A.
• Brittain, Derrick
• Froudarakis, Emmanouil
• Dorkenwald, Sven
• Collman, Forrest
• Kemnitz, Nico
• Ih, Dodam
• Silversmith, William M.
• Zung, Jonathan
• Zlateski, Aleksandar
• Tartavull, Ignacio
• Yu, Szi-chieh
• Popovych, Sergiy
• Mu, Shang
• Wong, William
• Jordan, Chris S.
• Castro, Manuel
• Buchanan, JoAnn
• Bumbarger, Daniel J.
• Takeno, Marc
• Torres, Russel
• Mahalingam, Gayathri
• Elabbady, Leila
• Li, Yang
• Cobos, Erick
• Zhou, Pengcheng
• Suckow, Shelby
• Becker, Lynne
• Paninski, Liam
• Polleux, Franck
• Reimer, Jacob
• Tolias, Andreas S.
• Reid, R. Clay
• da Costa, Nuno Maçarico
• Seung, H. Sebastian

Titel

Reconstruction of neocortex: Organelles, compartments, cells, circuits, and activity

Ist Teil von

• Cell, 2022-03, Vol.185 (6), p.1082-1100.e24

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2022

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• We assembled a semi-automated reconstruction of L2/3 mouse primary visual cortex from ∼250 × 140 × 90 μm3 of electron microscopic images, including pyramidal and non-pyramidal neurons, astrocytes, microglia, oligodendrocytes and precursors, pericytes, vasculature, nuclei, mitochondria, and synapses. Visual responses of a subset of pyramidal cells are included. The data are publicly available, along with tools for programmatic and three-dimensional interactive access. Brief vignettes illustrate the breadth of potential applications relating structure to function in cortical circuits and neuronal cell biology. Mitochondria and synapse organization are characterized as a function of path length from the soma. Pyramidal connectivity motif frequencies are predicted accurately using a configuration model of random graphs. Pyramidal cells receiving more connections from nearby cells exhibit stronger and more reliable visual responses. Sample code shows data access and analysis. [Display omitted] •A cortical reconstruction describes neuron connectivity, function, and cell biology•Pyramidal areal synapse density appears invariant with distance from the soma•Analysis of connectivity motifs reexamines "non-randomness" in cortical networks•Pyramidal cells with more local connections give stronger and more reliable responses Reconstruction of mouse visual cortex provides capacity for quantitative characterization of organelles, compartments, cells, circuits, and activity and their interrelations.