Details

Autor(en) / Beteiligte

• Dénou, Adama
• Togola, Adiaratou
• Inngjerdingen, Kari Tvete
• Moussavi, Nastaran
• Rise, Frode
• Zou, Yuan Feng
• Dafam, Dalen G.
• Nep, Elijah I.
• Ahmed, Abubakar
• Alemika, Taiwo E.
• Diallo, Drissa
• Sanogo, Rokia
• Paulsen, Berit Smestad

Titel

Isolation, characterisation and complement fixation activity of acidic polysaccharides from Argemone mexicana used as antimalarials in Mali

Ist Teil von

• Pharmaceutical biology, 2022-12, Vol.60 (1), p.1278-1285

Ort / Verlag

England: Taylor & Francis

Erscheinungsjahr

2022

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Global studies on Argemone mexicana L. (Papaveraceae) traditionally used against malaria in Mali are limited to its low-mass compounds activities, and little information on its bioactive polysaccharides is available. This study determines the structure and the immunomodulatory activity of polysaccharides from aerial parts of A. mexicana. Acidic polysaccharides from this plant material named HMAmA1 and HMAmA2 were isolated from water extracts. Their monosaccharide composition was determined by gas chromatography. Glycosidic linkages were determined using GC-MS. NMR was also applied. The polymers were tested for effects on the human complement system in vitro at different doses. The monosaccharide composition showed that the two polysaccharides contained in different amounts the following monomers: arabinose, rhamnose, galactose, and galacturonic acid. Overall structural analysis showed the presence of a low ratio of 1,2-linked rhamnose compared to 1,4-linked galacturonic acid with arabinogalactans substituted on position 4 of rhamnose. NMR data showed the presence of galacturonans alternated by rhamnogalacturonans bearing arabinose and galactose units. α-Linkages were found for l-arabinose, l-rhamnose and d-galacturonic acid, while β-linkages were found for d-galactose. The two polysaccharides exhibited strong complement fixation activities, with HMAmA1 being the highest potent fraction. ICH 50 value of HMAmA1 was 5 µg/mL, compared to the control BPII being 15.9 µg/mL. Polysaccharides form A. mexicana presented a complement fixation effect. The complement system is an important part of the immune defense, and compounds acting on the cascade are of interest. Therefore, these polymers may be useful as immunodulatory agents.