Details

Autor(en) / Beteiligte

• Xu, Jun
• Liu, Yifang
• Li, Hongjie
• Tarashansky, Alexander J
• Kalicki, Colin H
• Hung, Ruei-Jiun
• Hu, Yanhui
• Comjean, Aram
• Kolluru, Sai Saroja
• Wang, Bo
• Quake, Stephen R
• Luo, Liqun
• McMahon, Andrew P
• Dow, Julian A T
• Perrimon, Norbert

Titel

Transcriptional and functional motifs defining renal function revealed by single-nucleus RNA sequencing

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 2022-06, Vol.119 (25), p.e2203179119

Ort / Verlag

United States: National Academy of Sciences

Erscheinungsjahr

2022

Quelle

MEDLINE

Beschreibungen/Notizen

• Recent advances in single-cell sequencing provide a unique opportunity to gain novel insights into the diversity, lineage, and functions of cell types constituting a tissue/organ. Here, we performed a single-nucleus study of the adult renal system, consisting of Malpighian tubules and nephrocytes, which shares similarities with the mammalian kidney. We identified 11 distinct clusters representing renal stem cells, stellate cells, regionally specific principal cells, garland nephrocyte cells, and pericardial nephrocytes. Characterization of the transcription factors specific to each cluster identified ( ) as playing a role in stem cell regeneration and ( ) in regulating glycogen and triglyceride metabolism. In addition, we identified a number of genes, including ( ), ( ), , and that are involved in regulating the unusual star shape of stellate cells. Importantly, the single-nucleus dataset allows visualization of the expression at the organ level of genes involved in ion transport and junctional permeability, providing a systems-level view of the organization and physiological roles of the tubules. Finally, a cross-species analysis allowed us to match the fly kidney cell types to mouse kidney cell types and planarian protonephridia, knowledge that will help the generation of kidney disease models. Altogether, our study provides a comprehensive resource for studying the fly kidney.