Details

Autor(en) / Beteiligte

• Sorbini, Monica
• Togliatto, Gabriele
• Mioli, Fiorenza
• Simonato, Erika
• Marro, Matteo
• Cappuccio, Margherita
• Arruga, Francesca
• Caorsi, Cristiana
• Mansouri, Morteza
• Magistroni, Paola
• Gambella, Alessandro
• Delsedime, Luisa
• Papotti, Mauro Giulio
• Solidoro, Paolo
• Albera, Carlo
• Boffini, Massimo
• Rinaldi, Mauro
• Amoroso, Antonio
• Vaisitti, Tiziana
• Deaglio, Silvia

Titel

Validation of a Simple, Rapid, and Cost-Effective Method for Acute Rejection Monitoring in Lung Transplant Recipients

Ist Teil von

• Transplant international, 2022-06, Vol.35, p.10546-10546

Ort / Verlag

Frontiers Media S.A

Erscheinungsjahr

2022

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Despite advances in immunosuppression therapy, acute rejection remains the leading cause of graft dysfunction in lung transplant recipients. Donor-derived cell-free DNA is increasingly being considered as a valuable biomarker of acute rejection in several solid organ transplants. We present a technically improved molecular method based on digital PCR that targets the mismatch between the recipient and donor at the HLA-DRB1 locus. Blood samples collected sequentially post-transplantation from a cohort of lung recipients were used to obtain proof-of-principle for the validity of the assay, correlating results with transbronchial biopsies and lung capacity tests. The results revealed an increase in dd-cfDNA during the first 2 weeks after transplantation related to ischemia-reperfusion injury (6.36 ± 5.36%, p < 0.0001). In the absence of complications, donor DNA levels stabilized, while increasing again during acute rejection episodes (7.81 ± 12.7%, p < 0.0001). Respiratory tract infections were also involved in the release of dd-cfDNA (9.14 ± 15.59%, p = 0.0004), with a positive correlation with C-reactive protein levels. Overall, the dd-cfDNA percentages were inversely correlated with the lung function values measured by spirometry. These results confirm the value of dd-cfDNA determination during post-transplant follow-up to monitor acute rejection in lung recipients, achieved using a rapid and inexpensive approach based on the HLA mismatch between donor and recipient.