Details

Autor(en) / Beteiligte

• Agarwal, Dilip Kumar
• Hunt, Andrew C.
• Shekhawat, Gajendra S.
• Carter, Lauren
• Chan, Sidney
• Wu, Kejia
• Cao, Longxing
• Baker, David
• Lorenzo-Redondo, Ramon
• Ozer, Egon A.
• Simons, Lacy M.
• Hultquist, Judd F.
• Jewett, Michael C.
• Dravid, Vinayak P.

Titel

Rapid and Sensitive Detection of Antigen from SARS-CoV‑2 Variants of Concern by a Multivalent Minibinder-Functionalized Nanomechanical Sensor

Ist Teil von

• Analytical chemistry (Washington), 2022-06, Vol.94 (23), p.8105-8109

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2022

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• New platforms for the rapid and sensitive detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern are urgently needed. Here we report the development of a nanomechanical sensor based on the deflection of a microcantilever capable of detecting the SARS-CoV-2 spike (S) glycoprotein antigen using computationally designed multivalent minibinders immobilized on a microcantilever surface. The sensor exhibits rapid (<5 min) detection of the target antigens down to concentrations of 0.05 ng/mL (362 fM) and is more than an order of magnitude more sensitive than an antibody-based cantilever sensor. Validation of the sensor with clinical samples from 33 patients, including 9 patients infected with the Omicron (BA.1) variant observed detection of antigen from nasopharyngeal swabs with cycle threshold (Ct) values as high as 39, suggesting a limit of detection similar to that of the quantitative reverse transcription polymerase chain reaction (RT-qPCR). Our findings demonstrate the use of minibinders and nanomechanical sensors for the rapid and sensitive detection of SARS-CoV-2 and potentially other disease markers.