Details

Autor(en) / Beteiligte

• Luan, Xingkun
• Wang, Haozheng
• Xiang, Zehong
• Zhao, Jiruo
• Feng, Ying
• Shi, Qiang
• Baijun Liu
• Gong, Yumei
• Wong, Shing-Chung
• Yin, Jinghua

Titel

Construction of K + responsive surface on SEBS to reduce the hemolysis of preserved erythrocytes

Ist Teil von

• RSC advances, 2019-02, Vol.9 (10), p.5251-5258

Ort / Verlag

England: Royal Society of Chemistry

Erscheinungsjahr

2019

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Hemolysis of stored erythrocytes is a big obstacle for the development of new plasticizer-free polymer containers. Hemolysis is mainly caused by cell membrane oxidation and cation leaks from the intracellular fluid during storage. To construct an anti-hemolytic surface for a plasticizer-free polymer, we fabricated 2- -α-d-glucopyranosyl-l-ascorbic acid (AA-2G)-loaded polycaprolactone (PCL)-crown ether micro/nanofibers on the surface of styrene- -(ethylene- -butylene)- -styrene (SEBS). Our strategy is based on the sensitive response of the crown ether to leaked potassium, causing the release of AA-2G, the AA-2G can then remove the excess ROS, maintaining the Na/K-pump activity and the cell integrity. We demonstrated that the PCL-crown ether micro/nanofibers have been well prepared on the surface of SEBS; the micro/nanofibers provide a sensitive response to excess K and trigger the rapid release of AA-2G. AA-2G then acts as an antioxidant to reduce the excess ROS and maintain the Na/K-pump activity to mitigate cation leaks, resulting in the reduced hemolysis of the preserved erythrocytes. Our work thus provides a novel method for the development of plasticizer-free polymers for the storage of erythrocytes, and has the potential to be used to fabricate long-term anti-hemolytic biomaterials for use.