Details

Autor(en) / Beteiligte

• Şencan, İkbal
• Esipova, Tatiana
• Kılıç, Kıvılcım
• Li, Baoqiang
• Desjardins, Michèle
• Yaseen, Mohammad A
• Wang, Hui
• Porter, Jason E
• Kura, Sreekanth
• Fu, Buyin
• Secomb, Timothy W
• Boas, David A
• Vinogradov, Sergei A
• Devor, Anna
• Sakadžić, Sava

Titel

Optical measurement of microvascular oxygenation and blood flow responses in awake mouse cortex during functional activation

Ist Teil von

• Journal of cerebral blood flow and metabolism, 2022-03, Vol.42 (3), p.510-525

Ort / Verlag

London, England: SAGE Publications

Erscheinungsjahr

2022

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• The cerebral cortex has a number of conserved morphological and functional characteristics across brain regions and species. Among them, the laminar differences in microvascular density and mitochondrial cytochrome c oxidase staining suggest potential laminar variability in the baseline O2 metabolism and/or laminar variability in both O2 demand and hemodynamic response. Here, we investigate the laminar profile of stimulus-induced intravascular partial pressure of O2 (pO2) transients to stimulus-induced neuronal activation in fully awake mice using two-photon phosphorescence lifetime microscopy. Our results demonstrate that stimulus-induced changes in intravascular pO2 are conserved across cortical layers I–IV, suggesting a tightly controlled neurovascular response to provide adequate O2 supply across cortical depth. In addition, we observed a larger change in venular O2 saturation (ΔsO2) compared to arterioles, a gradual increase in venular ΔsO2 response towards the cortical surface, and absence of the intravascular “initial dip” previously reported under anesthesia. This study paves the way for quantification of layer-specific cerebral O2 metabolic responses, facilitating investigation of brain energetics in health and disease and informed interpretation of laminar blood oxygen level dependent functional magnetic resonance imaging signals.