Proceedings of the National Academy of Sciences - PNAS, 2022-03, Vol.119 (11), p.e2117245119-e2117245119
2022
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- Autor(en) / Beteiligte
- Titel
- FliL ring enhances the function of periplasmic flagella
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 2022-03, Vol.119 (11), p.e2117245119-e2117245119
- Ort / Verlag
- United States: National Academy of Sciences
- Erscheinungsjahr
- 2022
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- SignificanceHow flagella sense complex environments and control bacterial motility remain fascinating questions. Here, we deploy cryo-electron tomography to determine in situ structures of the flagellar motor in wild-type and mutant cells of , revealing that three flagellar proteins (FliL, MotA, and MotB) form a unique supramolecular complex in situ. Importantly, FliL not only enhances motor function by forming a ring around the stator complex MotA/MotB in its extended, active conformation but also facilitates assembly of the stator complex around the motor. Our in situ data provide insights into how cooperative remodeling of the FliL-stator supramolecular complex helps regulate the collective ion flux and establishes the optimal function of the flagellar motor to guide bacterial motility in various environments.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0027-8424eISSN: 1091-6490DOI: 10.1073/pnas.2117245119Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8931381
- Format
- –
- Schlagworte
- Bacteria, Bacterial Physiological Phenomena, Bacterial Proteins - chemistry, Bacterial Proteins - genetics, Bacterial Proteins - metabolism, Bacterial Proteins - ultrastructure, Biological Sciences, Borrelia burgdorferi, Conformation, Environmental conditions, Flagella, Flagella - metabolism, Flagella - ultrastructure, Gene Expression Regulation, Bacterial, Ions, Lyme disease, Membrane Proteins - chemistry, Membrane Proteins - metabolism, Membrane Proteins - ultrastructure, Models, Biological, Models, Molecular, Molecular Motor Proteins - genetics, Molecular Motor Proteins - metabolism, Motility, Motor stators, Oligomerization, Periplasm - metabolism, Periplasm - ultrastructure, Rotary machines, Spirochetes, Stators, Torque, Vector-borne diseases