Details

Autor(en) / Beteiligte

• Martín Ramírez, Alexandra
• Zurita Cruz, Nelly Daniela
• Gutiérrez-Cobos, Ainhoa
• Rodríguez Serrano, Diego Aníbal
• González Álvaro, Isidoro
• Roy Vallejo, Emilia
• Gómez de Frutos, Sara
• Fontán García-Rodrigo, Leticia
• Cardeñoso Domingo, Laura

Titel

Evaluation of two RT-PCR techniques for SARS-CoV-2 RNA detection in serum for microbiological diagnosis

Ist Teil von

• Journal of virological methods, 2022-02, Vol.300, p.114411-114411, Article 114411

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2022

Quelle

MEDLINE

Beschreibungen/Notizen

• •Cobas® and TaqPath™ kits are useful in SARS-CoV-2 detection in serum samples.•Cobas® and TaqPath™ kits are easily implemented in the microbiology laboratory.•SARS-CoV-2 detection in serum is more frequent in severe COVID-19 patients. Presence of SARS-CoV-2 RNA in serum (viremia) of COVID-19 patients has been related to poor prognosis and death. The aim of this study was to evaluate both the ability to detect viremia in COVID-19 patients of two commercial reverse real-time-PCR (rRT-PCR) tests, Cobas® and TaqPath™, comparing them with a gold standard method, and their implementation in microbiology laboratories. This retrospective cohort study included 303 adult patients (203 diagnosed with COVID-19 and 100 non-COVID-19 patients) admitted to a tertiary hospital, with at least one serum sample collected within the first 48 h from admission. A total of 365 serum samples were included: 100 from non-COVID patients (pre-pandemic and pandemic control groups) and 265 from COVID-19 patients. Serum samples were considered positive when at least one target was detected. All patients in control groups showed negative viremia. Cobas® and TaqPath™ tests showed specificity and Positive Predictive Value over 96%. Nevertheless, sensitivity (53.72 and 73.63, respectively) and Negative Predictive Value (64.78 and 75) were lower. Viremia difference between ICU and non-ICU patients was significant (p ≤ 0.001) for both techniques. Consequently, SARS-CoV-2 viremia detection by both rRT-PCR tests should be considered a good tool to stratify COVID-19 patients and could be implemented in microbiology laboratories.