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Periodontitis regulates renal impairment in obese mice via TGF-β/Smad pathway
Ist Teil von
American journal of translational research, 2021-01, Vol.13 (11), p.12523-12535
Ort / Verlag
United States: e-Century Publishing Corporation
Erscheinungsjahr
2021
Link zum Volltext
Quelle
Electronic Journals Library
Beschreibungen/Notizen
To determine the impact of periodontitis on renal impairment induced by obesity.
Periodontitis and obesity models were induced using silk ligatures with bacteria and high-fat diet, respectively. Indicators of renal function were compared. Renal tubular epithelial cells (RTECs) were treated with lipopolysaccharides from periodontal pathogens in a high-fat environment to induce cell models of periodontitis and obesity. The transforming growth factor-β/mothers against decapentaplegic homolog (Smad) (TGF-β/Smad) pathway was evaluated both
and
. The indicators of renal function, renal pathological changes, and serum inflammatory cytokines were measured. The viability/apoptosis of RTECs and the expression of inflammatory cytokines were determined.
Periodontitis resulted in an increase in TGF-β/Smad activity in the kidney of obese mice. Moreover, the activity of RTECs was also increased
. Downregulation of TGF-β led to reduced TGF-β, p-Smad2, p-Smad3, and Smad7 levels in kidney tissue and RTECs, ameliorated renal function indicators and renal pathological changes, increased viability and apoptosis of RTECs, and decreased levels of inflammatory cytokines.
Periodontitis regulates renal impairment via the TGF-β/Smad pathway in obese mice.