Details
- Autor(en) / Beteiligte
- Titel
- Multi‐miRNA panel of tumor‐derived extracellular vesicles as promising diagnostic biomarkers of early‐stage breast cancer
- Ist Teil von
- Cancer science, 2021-12, Vol.112 (12), p.5078-5087
- Ort / Verlag
- England: John Wiley & Sons, Inc
- Erscheinungsjahr
- 2021
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Extracellular vesicles (EV) have been emerging as potential biomarkers for disease monitoring. In particular, tumor‐derived EV (TDE) are known to carry oncogenic miRNA, so they can be used for diagnosis of early cancer by analyzing the expression levels of EV‐miRNA circulating in the blood. Here, using our novel microfluidic device, we rapidly and selectively isolate cancerous EV expressing breast cancer‐derived surface markers CD49f and EpCAM within 2 minutes. Based on seven candidates of miRNA nominated from The Cancer Genome Atlas (TCGA) database, the expression levels of miRNA in TDE were validated in a total of 82 individuals, including 62 breast cancer patients and 20 healthy controls. Among seven candidates, four miRNAs (miR‐9, miR‐16, miR‐21, and miR‐429) from the EV were highly elevated in early‐stage breast cancer patients compared with healthy donors. The combination of significant miRNAs from specific EV has high sensitivities of 0.90, 0.86, 0.88, and 0.84 of the area under the receiver operating characteristic curve (AUC) in each subtype (luminal A, luminal B, HER‐2, and triple‐negative) of early‐stage breast cancer. Our results suggest that the combination of four miRNA signatures of specific EV could serve as a sensitive and specific biomarker and enable early diagnosis of breast cancer using liquid biopsy. A new combination of miRNA from cancer‐specific extracellular vesicles was proposed for the highly sensitive and specific diagnosis of early‐stage breast cancer in association with a microfluidic‐based immuno‐isolation technique. These findings suggest that miRNA in EV could be used as important biomarkers for the early detection of breast cancer and identification of cancer subtype.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1347-9032eISSN: 1349-7006DOI: 10.1111/cas.15155Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8645748
- Format
- –
- Schlagworte
- biomarker, Biomarkers, Biomarkers, Tumor - genetics, Biopsy, Breast cancer, Breast Neoplasms - diagnosis, Breast Neoplasms - genetics, Breast Neoplasms - pathology, Case-Control Studies, Cell Line, Tumor, Databases, Genetic, Diagnosis, Early Detection of Cancer, early‐stage breast cancer, Epithelial Cell Adhesion Molecule - metabolism, Extracellular vesicles, Extracellular Vesicles - genetics, Extracellular Vesicles - metabolism, Female, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Genomes, Humans, Integrin alpha6 - metabolism, liquid biopsy, MCF-7 Cells, Medical diagnosis, Microfluidic Analytical Techniques - instrumentation, Microfluidics, microRNA, MicroRNAs, MicroRNAs - genetics, miRNA, Neoplasm Staging, Original, Plasma, Proteins, Surface markers, Tumors, Up-Regulation