Biochemistry (Moscow), 2021-11, Vol.86 (11), p.1469-1476
2021
Details
- Autor(en) / Beteiligte
- Titel
- Monoclonal Antibody Targeting the HA191/199 Region of H1N1 Influenza Virus Mediates the Damage of Neural Cells
- Ist Teil von
- Biochemistry (Moscow), 2021-11, Vol.86 (11), p.1469-1476
- Ort / Verlag
- Moscow: Pleiades Publishing
- Erscheinungsjahr
- 2021
- Link zum Volltext
- Quelle
- springer (창간호~2014)
- Beschreibungen/Notizen
- Vaccination is the most effective mean of preventing influenza virus infections. However, vaccination-induced adverse reactions of the nervous system, the causes of which are unknown, lead to concerns on the safety of influenza A vaccine. In this study, we used flow cytometry, cell ELISA, and immunofluorescence to find that H1-84 monoclonal antibody (mAb) against the191/199 region of the H1N1 influenza virus hemagglutinin (HA) protein binds to neural cells and mediates cell damage. Using molecular simulation software, such as PyMOL and PDB viewer, we demonstrated that the HA191/199 region maintains the overall structure of the HA head. Since the HA191/199 region cannot be removed from the HA structure, it has to be altered via introducing point mutations by site-directed mutagenesis. This will provide an innovative theoretical support for the subsequent modification the influenza A vaccine for increasing its safety.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0006-2979eISSN: 1608-3040DOI: 10.1134/S0006297921110109Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8588936
- Format
- –
- Schlagworte
- Antibodies, Monoclonal, Murine-Derived - chemistry, Antibodies, Monoclonal, Murine-Derived - immunology, Antibodies, Viral - chemistry, Antibodies, Viral - immunology, Biochemistry, Biomedical and Life Sciences, Biomedicine, Bioorganic Chemistry, Cell Line, Tumor, Complications and side effects, Damage, Enzyme-linked immunosorbent assay, Flow cytometry, Hemagglutinin Glycoproteins, Influenza Virus - chemistry, Hemagglutinin Glycoproteins, Influenza Virus - genetics, Hemagglutinin Glycoproteins, Influenza Virus - immunology, Hemagglutinins, Humans, Immunization, Immunofluorescence, Influenza, Influenza A, Influenza A Virus, H1N1 Subtype - chemistry, Influenza A Virus, H1N1 Subtype - genetics, Influenza A Virus, H1N1 Subtype - immunology, Influenza vaccines, Influenza viruses, Lectins, Life Sciences, Medical research, Medicine, Experimental, Microbiology, Molecular Dynamics Simulation, Monoclonal antibodies, Mutagenesis, Site-Directed, Mutation, Nervous system, Neurons - metabolism, Neurons - pathology, Protein binding, Protein Domains, Safety, Site-directed mutagenesis, Software, Swine flu, Swine influenza, Vaccination, Vaccines, Viruses