Details

Autor(en) / Beteiligte

• Ge, Luandie
• Fan, Yaqi
• Fu, Lin
• Guo, Mengjiao
• Cao, Panxia
• Peng, Chaojie
• Wu, Linke
• Han, Lihua
• Wu, Hong
• Wang, Qi-Rui
• Qi-Rui Wang

Titel

YQHX Alleviates H/R-Induced Cardiomyocyte Apoptosis by Downregulating miR-1

Ist Teil von

• Evidence-based complementary and alternative medicine, 2021-11, Vol.2021, p.1-9

Ort / Verlag

New York: Hindawi

Erscheinungsjahr

2021

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Yiqi Huoxue granule (YQHX) inhibits cardiomyocyte apoptosis in myocardial ischemia-reperfusion injury (MIRI); however, the underlying mechanism is unknown. In this study, hypoxia-reoxygenation (H/R) models were established using rat myocardial primary cells and H9c2 cells, lactate dehydrogenase (LDH), and creatine kinase (CK) levels and cardiomyocyte apoptosis were determined. LDH release, CK activity, caspase-3 activation, mRNA and protein ratio of Bax/Bcl-2, and miR-1 expression were significantly higher (p<0.01) in the H/R model of rat myocardial primary cells and H9c2 cells compared with the control group and was inhibited by YQHX treatment (p<0.01 or p<0.05). We also found that miR-1 overexpression could enhance apoptosis in cardiomyocytes, whereas apoptosis could be reduced by YQHX treatment (p<0.01). In conclusion, YQHX alleviates H/R-induced cardiomyocyte apoptosis by inhibiting miR-1 expression, suggesting the potential of YQHX in preventing MIRI.