Details

Autor(en) / Beteiligte

• Isaka, Masanori
• Okamoto, Akira
• Miura, Yutaka
• Tatsuno, Ichiro
• Maeyama, Jun-Ichi
• Hasegawa, Tadao
• Freitag, Nancy E
• Freitag, Nancy E.

Titel

Streptococcus pyogenes TrxSR Two-Component System Regulates Biofilm Production in Acidic Environments

Ist Teil von

• Infection and immunity, 2021-10, Vol.89 (11), p.e0036021

Ort / Verlag

United States: American Society for Microbiology

Erscheinungsjahr

2021

Quelle

MEDLINE

Beschreibungen/Notizen

• Bacteria form biofilms for their protection against environmental stress and produce virulence factors within the biofilm. Biofilm formation in acidified environments is regulated by a two-component system, as shown by studies on isogenic mutants of the sensor protein of the two-component regulatory system in Streptococcus pyogenes. In this study, we found that the LiaS histidine kinase sensor mediates biofilm production and pilus expression in an acidified environment through glucose fermentation. The isogenic mutant produced biofilms in a culture acidified by hydrochloric acid but not glucose, suggesting that the acidified environment is sensed by another protein. In addition, the isogenic mutant could not produce biofilms or activate the promoter in an acidified environment. Mass spectrometry analysis showed that TrxS regulates M protein, consistent with the transcriptional regulation of , which encodes M protein. Our results demonstrate that biofilm production during environmental acidification is directly under the control of TrxS.