Details

Autor(en) / Beteiligte

• Neve, Rachel L
• Carrillo, Brent D
• Phelan, Vanessa V
• O'Toole, George

Titel

Impact of Artificial Sputum Medium Formulation on Pseudomonas aeruginosa Secondary Metabolite Production

Ist Teil von

• Journal of bacteriology, 2021-10, Vol.203 (21), p.e0025021-e0025021

Ort / Verlag

United States: American Society for Microbiology

Erscheinungsjahr

2021

Quelle

MEDLINE

Beschreibungen/Notizen

• culture media are being developed to understand how host site-specific nutrient profiles influence microbial pathogenicity and ecology. To mimic the cystic fibrosis (CF) lung environment, a variety of artificial sputum media (ASM) have been created. However, the composition of these ASM vary in the concentration of key nutrients, including amino acids, lipids, DNA, and mucin. In this work, we used feature-based molecular networking (FBMN) to perform comparative metabolomics of Pseudomonas aeruginosa, the predominant opportunistic pathogen infecting the lungs of people with CF, cultured in nine different ASM. We found that the concentration of aromatic amino acids and iron from mucin added to the media contributes to differences in the production of P. aeruginosa virulence-associated secondary metabolites. Different media formulations aiming to replicate infection environments contain different nutrients, which affects interpretation of experimental results. Inclusion of undefined components, such as commercial porcine gastric mucin (PGM), in an otherwise chemically defined medium can alter the nutrient content of the medium in unexpected ways and influence experimental outcomes.