Details

Autor(en) / Beteiligte

• Moraskie, Michael
• Roshid, Md Harun Or
• O'Connor, Gregory
• Dikici, Emre
• Zingg, Jean-Marc
• Deo, Sapna
• Daunert, Sylvia

Titel

Microbial whole-cell biosensors: Current applications, challenges, and future perspectives

Ist Teil von

• Biosensors & bioelectronics, 2021-11, Vol.191, p.113359-113359, Article 113359

Ort / Verlag

England: Elsevier B.V

Erscheinungsjahr

2021

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Microbial Whole-Cell Biosensors (MWCBs) have seen rapid development with the arrival of 21st century biological and technological capabilities. They consist of microbial species which produce, or limit the production of, a reporter protein in the presence of a target analyte. The quantifiable signal from the reporter protein can be used to determine the bioavailable levels of the target analyte in a variety of sample types at a significantly lower cost than most widely used and well-established analytical instrumentation. Furthermore, the versatile and robust nature of MWCBs shows great potential for their use in otherwise unavailable settings and environments. While MWCBs have been developed for use in biomedical, environmental, and agricultural monitoring, they still face various challenges before they can transition from the laboratory into industrialized settings like their enzyme-based counterparts. In this comprehensive and critical review, we describe the underlying working principles of MWCBs, highlight developments for their use in a variety of fields, detail challenges and current efforts to address them, and discuss exciting implementations of MWCBs helping redefine what is thought to be possible with this expeditiously evolving technology. •Microbial whole-cell biosensors (MWCBs) detect analytes relevant to agricultural, environmental, and medical applications.•Work is currently underway to address major challenges with their safe deployment, analytical performance, and shelf-life.•MWCBs are highly adaptable for their incorporation with emerging technologies such as nanomaterials and autonomous devices.