Details

Autor(en) / Beteiligte

• Smits, Nathan
• Rasmussen, Jay
• Bodea, Gabriela O.
• Amarilla, Alberto A.
• Gerdes, Patricia
• Sanchez-Luque, Francisco J.
• Ajjikuttira, Prabha
• Modhiran, Naphak
• Liang, Benjamin
• Faivre, Jamila
• Deveson, Ira W.
• Khromykh, Alexander A.
• Watterson, Daniel
• Ewing, Adam D.
• Faulkner, Geoffrey J.

Titel

No evidence of human genome integration of SARS-CoV-2 found by long-read DNA sequencing

Ist Teil von

• Cell reports (Cambridge), 2021-08, Vol.36 (7), p.109530-109530, Article 109530

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2021

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• A recent study proposed that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) hijacks the LINE-1 (L1) retrotransposition machinery to integrate into the DNA of infected cells. If confirmed, this finding could have significant clinical implications. Here, we apply deep (>50×) long-read Oxford Nanopore Technologies (ONT) sequencing to HEK293T cells infected with SARS-CoV-2 and do not find the virus integrated into the genome. By examining ONT data from separate HEK293T cultivars, we completely resolve 78 L1 insertions arising in vitro in the absence of L1 overexpression systems. ONT sequencing applied to hepatitis B virus (HBV)-positive liver cancer tissues located a single HBV insertion. These experiments demonstrate reliable resolution of retrotransposon and exogenous virus insertions by ONT sequencing. That we find no evidence of SARS-CoV-2 integration suggests that such events are, at most, extremely rare in vivo and therefore are unlikely to drive oncogenesis or explain post-recovery detection of the virus. [Display omitted] •Nanopore sequencing of SARS-CoV-2-infected HEK293T cells and control samples•No SARS-CoV-2 genomic integrants were found in any sample•LINE-1 insertions carrying retrotransposition hallmarks were detected in HEK293T cells•Hepatitis B virus integration was observed by sequencing liver cancer samples In response to a recent claim that SARS-CoV-2 can be incorporated into human DNA by LINE-1 retrotransposon proteins, Smits et al. apply long-read DNA sequencing to cultured HEK293T cells infected with SARS-CoV-2. They find no evidence for genomic integration of the virus, despite demonstrated availability of the LINE-1 protein machinery.