Details

Autor(en) / Beteiligte

• Opstelten, Rianne
• Suwandi, Jessica S
• Slot, Manon C.
• Morgana, Florencia
• Scott, Andrew M
• Laban, Sandra
• Nikolic, Tatjana
• Turksma, Annelies W
• Kroeze, Anna
• Voermans, Carlijn
• Zwaginga, Jaap‐Jan
• Roep, Bart O
• Amsen, Derk

Titel

GPA33 is expressed on multiple human blood cell types and distinguishes CD4+ central memory T cells with and without effector function

Ist Teil von

• European journal of immunology, 2021-06, Vol.51 (6), p.1377-1389

Ort / Verlag

Germany: Wiley Subscription Services, Inc

Erscheinungsjahr

2021

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• The Ig superfamily protein glycoprotein A33 (GPA33) has been implicated in immune dysregulation, but little is known about its expression in the immune compartment. Here, we comprehensively determined GPA33 expression patterns on human blood leukocyte subsets, using mass and flow cytometry. We found that GPA33 was expressed on fractions of B, dendritic, natural killer and innate lymphoid cells. Most prominent expression was found in the CD4+ T cell compartment. Naïve and CXCR5+ regulatory T cells were GPA33high, and naïve conventional CD4+ T cells expressed intermediate GPA33 levels. The expression pattern of GPA33 identified functional heterogeneity within the CD4+ central memory T cell (Tcm) population. GPA33+ CD4+ Tcm cells were fully undifferentiated, bona fide Tcm cells that lack immediate effector function, whereas GPA33– Tcm cells exhibited rapid effector functions and may represent an early stage of differentiation into effector/effector memory T cells before loss of CD62L. Expression of GPA33 in conventional CD4+ T cells suggests a role in localization and/or preservation of an undifferentiated state. These results form a basis to study the function of GPA33 and show it to be a useful marker to discriminate between different cellular subsets, especially in the CD4+ T cell lineage. Among human blood leukocytes GPA33 is predominantly expressed on CD4+ T cells that lack effector function. GPA33 marks naïve CD4+ T cells and separates the CCR7+CD62L+ central memory compartment into an undifferentiated population and one that exhibits effector properties, suggesting GPA33 has a role in localization and/or preservation of quiescence.