Details

Autor(en) / Beteiligte

• Tarke, Alison
• Sidney, John
• Methot, Nils
• Yu, Esther Dawen
• Zhang, Yun
• Dan, Jennifer M.
• Goodwin, Benjamin
• Rubiro, Paul
• Sutherland, Aaron
• Wang, Eric
• Frazier, April
• Ramirez, Sydney I.
• Rawlings, Stephen A.
• Smith, Davey M.
• da Silva Antunes, Ricardo
• Peters, Bjoern
• Scheuermann, Richard H.
• Weiskopf, Daniela
• Crotty, Shane
• Grifoni, Alba
• Sette, Alessandro

Titel

Impact of SARS-CoV-2 variants on the total CD4+ and CD8+ T cell reactivity in infected or vaccinated individuals

Ist Teil von

• Cell reports. Medicine, 2021-07, Vol.2 (7), p.100355-100355, Article 100355

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Electronic Journals Library

Beschreibungen/Notizen

• The emergence of SARS-CoV-2 variants with evidence of antibody escape highlight the importance of addressing whether the total CD4+ and CD8+ T cell recognition is also affected. Here, we compare SARS-CoV-2-specific CD4+ and CD8+ T cells against the B.1.1.7, B.1.351, P.1, and CAL.20C lineages in COVID-19 convalescents and in recipients of the Moderna (mRNA-1273) or Pfizer/BioNTech (BNT162b2) COVID-19 vaccines. The total reactivity against SARS-CoV-2 variants is similar in terms of magnitude and frequency of response, with decreases in the 10%–22% range observed in some assay/VOC combinations. A total of 7% and 3% of previously identified CD4+ and CD8+ T cell epitopes, respectively, are affected by mutations in the various VOCs. Thus, the SARS-CoV-2 variants analyzed here do not significantly disrupt the total SARS-CoV-2 T cell reactivity; however, the decreases observed highlight the importance for active monitoring of T cell reactivity in the context of SARS-CoV-2 evolution. [Display omitted] T cells of exposed donors or vaccinees effectively recognize SARS-CoV-2 variantsEffective recognition in AIM and FluoroSPOT assays, for spike and other proteins93% and 97% of CD4 and CD8 epitopes are 100% conserved across variants Tarke et al. show that SARS-CoV-2-specific memory CD4 and CD8 T cells exposed to the ancestral strain by infection or vaccination effectively recognize the variants B.1.1.7, B.1.351, P.1, and CAL.20C. The majority of T cell epitopes are unaffected by mutations in these variant strains.