Bio-protocol, 2018-02, Vol.8 (4), p.e2736-e2736
2018
Details
- Autor(en) / Beteiligte
- Titel
- Assaying Mechanonociceptive Behavior in Drosophila Larvae
- Ist Teil von
- Bio-protocol, 2018-02, Vol.8 (4), p.e2736-e2736
- Ort / Verlag
- United States: Bio-Protocol
- Erscheinungsjahr
- 2018
- Link zum Volltext
- Quelle
- EZB Electronic Journals Library
- Beschreibungen/Notizen
- larvae have been extensively used as a model to study the molecular and cellular basis of nociception. The larval nociceptors, class IV dendritic arborization (C4da) neurons, line the body wall of the animal and respond to various stimuli including noxious heat and touch. Activation of C4da neurons results in a stereotyped escape behavior, characterized by a 360° rolling response along the body axis followed by locomotion speedup. The genetic accessibility of has allowed the identification of mechanosensory channels and circuit elements required for nociceptive responses, making it a useful and straightforward readout to understand the cellular and molecular basis of nociceptive function and behavior. We have optimized the protocol to assay mechanonociceptive behavior in larvae.