Details

Autor(en) / Beteiligte

• Low, Teck Yew
• Syafruddin, Saiful Effendi
• Mohtar, M. Aiman
• Vellaichamy, Adaikkalam
• A Rahman, Nisa Syakila
• Pung, Yuh-Fen
• Tan, Chris Soon Heng

Titel

Recent progress in mass spectrometry-based strategies for elucidating protein–protein interactions

Ist Teil von

• Cellular and molecular life sciences : CMLS, 2021-07, Vol.78 (13), p.5325-5339

Ort / Verlag

Cham: Springer International Publishing

Erscheinungsjahr

2021

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Protein–protein interactions are fundamental to various aspects of cell biology with many protein complexes participating in numerous fundamental biological processes such as transcription, translation and cell cycle. MS-based proteomics techniques are routinely applied for characterising the interactome, such as affinity purification coupled to mass spectrometry that has been used to selectively enrich and identify interacting partners of a bait protein. In recent years, many orthogonal MS-based techniques and approaches have surfaced including proximity-dependent labelling of neighbouring proteins, chemical cross-linking of two interacting proteins, as well as inferring PPIs from the co-behaviour of proteins such as the co-fractionating profiles and the thermal solubility profiles of proteins. This review discusses the underlying principles, advantages, limitations and experimental considerations of these emerging techniques. In addition, a brief account on how MS-based techniques are used to investigate the structural and functional properties of protein complexes, including their topology, stoichiometry, copy number and dynamics, are discussed.