Details

Autor(en) / Beteiligte

• Stephenson, Emily
• Reynolds, Gary
• Botting, Rachel A.
• Calero-Nieto, Fernando J.
• Morgan, Michael D.
• Tuong, Zewen Kelvin
• Bach, Karsten
• Sungnak, Waradon
• Worlock, Kaylee B.
• Yoshida, Masahiro
• Kumasaka, Natsuhiko
• Kania, Katarzyna
• Engelbert, Justin
• Olabi, Bayanne
• Spegarova, Jarmila Stremenova
• Wilson, Nicola K.
• Mende, Nicole
• Jardine, Laura
• Gardner, Louis C. S.
• Goh, Issac
• Horsfall, Dave
• McGrath, Jim
• Webb, Simone
• Mather, Michael W.
• Lindeboom, Rik G. H.
• Dann, Emma
• Huang, Ni
• Polanski, Krzysztof
• Prigmore, Elena
• Gothe, Florian
• Scott, Jonathan
• Payne, Rebecca P.
• Baker, Kenneth F.
• Hanrath, Aidan T.
• Schim van der Loeff, Ina C. D.
• Barr, Andrew S.
• Sanchez-Gonzalez, Amada
• Bergamaschi, Laura
• Mescia, Federica
• Barnes, Josephine L.
• Kilich, Eliz
• de Wilton, Angus
• Saigal, Anita
• Saleh, Aarash
• Janes, Sam M.
• Smith, Claire M.
• Gopee, Nusayhah
• Wilson, Caroline
• Coupland, Paul
• Coxhead, Jonathan M.
• Kiselev, Vladimir Yu
• van Dongen, Stijn
• Bacardit, Jaume
• King, Hamish W.
• Rostron, Anthony J.
• Simpson, A. John
• Hambleton, Sophie
• Laurenti, Elisa
• Lyons, Paul A.
• Meyer, Kerstin B.
• Nikolić, Marko Z.
• Duncan, Christopher J. A.
• Smith, Kenneth G. C.
• Teichmann, Sarah A.
• Clatworthy, Menna R.
• Marioni, John C.
• Göttgens, Berthold
• Haniffa, Muzlifah

Titel

Single-cell multi-omics analysis of the immune response in COVID-19

Ist Teil von

• Nature medicine, 2021-05, Vol.27 (5), p.904-916

Ort / Verlag

New York: Nature Publishing Group US

Erscheinungsjahr

2021

Quelle

MEDLINE

Beschreibungen/Notizen

• Analysis of human blood immune cells provides insights into the coordinated response to viral infections such as severe acute respiratory syndrome coronavirus 2, which causes coronavirus disease 2019 (COVID-19). We performed single-cell transcriptome, surface proteome and T and B lymphocyte antigen receptor analyses of over 780,000 peripheral blood mononuclear cells from a cross-sectional cohort of 130 patients with varying severities of COVID-19. We identified expansion of nonclassical monocytes expressing complement transcripts ( CD16 + C1QA/B/C + ) that sequester platelets and were predicted to replenish the alveolar macrophage pool in COVID-19. Early, uncommitted CD34 + hematopoietic stem/progenitor cells were primed toward megakaryopoiesis, accompanied by expanded megakaryocyte-committed progenitors and increased platelet activation. Clonally expanded CD8 + T cells and an increased ratio of CD8 + effector T cells to effector memory T cells characterized severe disease, while circulating follicular helper T cells accompanied mild disease. We observed a relative loss of IgA2 in symptomatic disease despite an overall expansion of plasmablasts and plasma cells. Our study highlights the coordinated immune response that contributes to COVID-19 pathogenesis and reveals discrete cellular components that can be targeted for therapy. Transcriptomic and proteomic profiling of blood samples from individuals with COVID-19 reveals immune cell and hematopoietic progenitor cell alterations that are differentially associated with disease severity.