Details

Autor(en) / Beteiligte

• Beesley, Nicola J.
• Attree, Elizabeth
• Vázquez-Prieto, Severo
• Vilas, Román
• Paniagua, Esperanza
• Ubeira, Florencio M.
• Jensen, Oscar
• Pruzzo, Cesar
• Álvarez, José D.
• Malandrini, Jorge Bruno
• Solana, Hugo
• Hodgkinson, Jane E.

Titel

Evidence of population structuring following population genetic analyses of Fasciola hepatica from Argentina

Ist Teil von

• International journal for parasitology, 2021-05, Vol.51 (6), p.471-480

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2021

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• [Display omitted] •320 Argentinian Fasciola hepatica were genotyped using a panel of microsatellites.•Overall there was high genotypic richness: 263 distinct genotypes were identified.•Population structuring of F. hepatica was evident across Argentina.•Within these sub-populations there is largely random mating.•Transmission of clonemates occurs: clonal parasites accounted for 26.6% of all parasites. Fasciola hepatica, the liver fluke, is a trematode parasite that causes disease of economic importance in livestock. As a zoonosis this parasite also poses a risk to human health in areas where it is endemic. Population genetic studies can reveal the mechanisms responsible for genetic structuring (non-panmixia) within parasite populations and provide valuable insights into population dynamics, which in turn enables theoretical predictions of evolutionary dynamics such as the evolution of drug resistance. Here we genotyped 320 F. hepatica collected from 14 definitive hosts from four provinces in Argentina. STRUCTURE analysis indicated three population clusters, and principal coordinate analysis confirmed this, showing population clustering across provinces. Similarly, pairwise FST values amongst all four provinces were significant, with standardised pairwise FST (F′ST) ranging from 0.0754 to 0.6327. Therefore, population genetic structure was evident across these four provinces in Argentina. However, there was no evidence of deviation from Hardy–Weinberg equilibrium, so it appears that within these sub-populations there is largely random mating. We identified 263 unique genotypes, which gave a clonal diversity of 82%. Parasites with identical genotypes, clones, accounted for 26.6% of the parasites studied and were found in 12 of the 14 hosts studied, suggesting some clonemate transmission.