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MIG-6 is essential for promoting glucose metabolic reprogramming and tumor growth in triple-negative breast cancer
Ist Teil von
EMBO reports, 2021-05, Vol.22 (5), p.e50781-n/a
Ort / Verlag
London: Nature Publishing Group UK
Erscheinungsjahr
2021
Quelle
Wiley Online Library Journals Frontfile Complete
Beschreibungen/Notizen
Treatment of triple-negative breast cancer (TNBC) remains challenging due to a lack of effective targeted therapies. Dysregulated glucose uptake and metabolism are essential for TNBC growth. Identifying the molecular drivers and mechanisms underlying the metabolic vulnerability of TNBC is key to exploiting dysregulated cancer metabolism for therapeutic applications. Mitogen-inducible gene-6 (MIG-6) has long been thought of as a feedback inhibitor that targets activated EGFR and suppresses the growth of tumors driven by constitutive activated mutant EGFR. Here, our bioinformatics and histological analyses uncover that MIG-6 is upregulated in TNBC and that MIG-6 upregulation is positively correlated with poorer clinical outcomes in TNBC. Metabolic arrays and functional assays reveal that MIG-6 drives glucose metabolism reprogramming toward glycolysis. Mechanistically, MIG-6 recruits HAUSP deubiquitinase for stabilizing HIF1α protein expression and the subsequent upregulation of GLUT1 and other HIF1α-regulated glycolytic genes, substantiating the comprehensive regulation of MIG-6 in glucose metabolism. Moreover, our mouse studies demonstrate that MIG-6 regulates GLUT1 expression in tumors and subsequent tumor growth
in vivo
. Collectively, this work reveals that MIG-6 is a novel prognosis biomarker, metabolism regulator, and molecular driver of TNBC.
SYNOPSIS
MIG-6 is upregulated in TNBC and promotes tumor growth by fueling glycolytic flux via the HIf1α-Glut1 pathway.
MIG-6 levels correlate with disease progression and bad prognosis in TNBC patients.
Depletion of MIG-6 restricts TNBC cell proliferation and TNBC xenograft growth in mice.
MIG-6 promotes aerobic glycolysis by promoting GLUT1 transcription.
MIG-6 recruits HAUSP deubiquitinase for stabilizing HIF1α protein, which in turn upregulates GLUT1.
Graphical Abstract
MIG-6 is upregulated in TNBC and promotes tumor growth by fueling glycolytic flux via the HIf1α-Glut1 pathway.