Details

Autor(en) / Beteiligte

• Rauch, Jennifer N
• Valois, Eric
• Solley, Sabrina C
• Braig, Friederike
• Lach, Ryan S
• Audouard, Morgane
• Ponce-Rojas, Jose Carlos
• Costello, Michael S
• Baxter, Naomi J
• Kosik, Kenneth S
• Arias, Carolina
• Acosta-Alvear, Diego
• Wilson, Maxwell Z
• Hayden, Randall

Titel

A Scalable, Easy-to-Deploy Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material

Ist Teil von

• Journal of clinical microbiology, 2021-03, Vol.59 (4)

Ort / Verlag

United States: American Society for Microbiology

Erscheinungsjahr

2021

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• The COVID-19 pandemic has created massive demand for widespread, distributed tools for detecting SARS-CoV-2 genetic material. The hurdles to scalable testing include reagent and instrument accessibility, availability of highly trained personnel, and large upfront investment. Here, we showcase an orthogonal pipeline we call CREST (Cas13-based, rugged, equitable, scalable testing) that addresses some of these hurdles. Specifically, CREST pairs commonplace and reliable biochemical methods (PCR) with low-cost instrumentation, without sacrificing detection sensitivity. By taking advantage of simple fluorescence visualizers, CREST allows a binary interpretation of results. CREST may provide a point-of-care solution to increase the distribution of COVID-19 surveillance.